Protocols provided for transfection of DNA mRNA siRNA and microRNA
Kit includes Transfection Enhancer reagent and recommended transfection protocols
Effective for plasmid DNA/siRNA co-transfection
Work in presence of serum
Ideal for primary cultures
Reproducible transfection
Cost-effective transfection
Low cytotoxicity
Developed and manufactured by Altogen Biosystems
Transfection Efficiency:
Reagent exhibits at least 70% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.
Transfection Protocol:
Transfection protocols are provided with the purchase of the reagent.
Shipping information:
US orders are shipped within 1-2 busines days via UPS Express.
International orders are shipped within 3-5 business days (Europe) and 4-9 business days (Worldwide).For maximum stability store all reagents at +4C upon receipt.
Figure 1. SiRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected following the recommended protocol. At 48 hours post-transfection the cells were analyzed by qRT-PCR for gene expression levels. 18S rRNA levels were used to normalize the Lamin A/C data. Values are normalized to untreated sample. Data are means ± SD (n=3).